JAX® Microinjection Service
DNA and embryonic stem (ES) cell microinjection from the mouse experts to produce custom genetically modified mice
NEW: DNA Microinjection Service now available into NOD/ShiLtJ embryos
DNA Microinjection Service
Key benefits
Extensive model development experience with well-established protocols
- DNA injection into your choice of C57BL/6J (000664), FVB/NJ (001800) or NOD/ShiLtJ (001976) embryos
- Minimum of three founders*
- Eliminates the time and expense of backcrossing
- All mice produced are specific and opportunistic pathogen-free (SOPF)
Customer-supplied materials
- At least 50µg of purified plasmid or supercoiled BAC DNA
- Map of the plasmid denoting required restriction enzyme sites
- An agarose gel image of the digested plasmid
- Expected phenotype (if any) and other pertinent information
Deliverables
- DNA preparation and injection into 250 embryos
- Delivery of all weaned mice, typically 50 or more*
- Project completion in 12 to 14 weeks
- All mice produced are SOPF
* If JAX performs the genotyping, we will provide a minimum of three or more founders or we will repeat injections at no additional cost.
ES Cell Microinjection Service
Key benefits
- Microinjection of gene-targeted ES cells into 100 C57BL/6J or B6(Cg)-Tyrc-2J/J (000058) embryos
- All mice produced are specific and opportunistic pathogen-free (SOPF)
Customer-supplied materials
- At least two frozen vials each containing six million or more gene-targeted 129 or C57BL/6 ES cells
- Appropriate cell background information (e.g. parental cell line, special culture conditions, expected phenotype) and other pertinent information
Deliverables
- Preparation of ES cells and microinjection into 100 C57BL/6J or B6(Cg)-Tyrc-2J/J embryos
- Mice produced scored for coat-color chimerism†
- Delivery of all chimeric mice produced and certified SOPF health report
- Project completion in 12 to 14 weeks
† Approximately 90% of our projects yield coat color chimeric mice.
JAX® Speed Expansion Service
JAX can quickly expand your new model using our Speed Expansion Service. All mice will be the same age and at a SOPF status. Using IVF, we routinely produce cohorts of mice that can be used to jump start a breeding colony or perform characterization studies. This saves months over traditional breeding approaches.