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JAX compound evaluation: inflammatory and autoimmune disease

The JAX—West testing facility specializes in compound evaluation using the most widely accepted models and readouts of human rheumatoid arthritis, multiple sclerosis (MS), asthma, type 1 diabetes and other inflammatory and autoimmune diseases:

• Asthma
• Experimental allergic encephalomyelitis
• Delayed type hypersensitivity
• Rheumatoid arthritis
• Inflammatory bowel disease
• Systemic Lupus Erythematosus
• Type 1 diabetes
• Graft-versus-host disease

Asthma

• Compound efficacy testing in an experimental asthma model
• Models:
  • BALB/cJ immunized with ovalbumin
  • BALB/cByJ mice with ovalbumin
• Readouts:
  • Pulmonary resistance by Penh
  • Inflammatory biomarkers
  • Histopathology
  • Sample study: OVA-Sensitization model of Asthma in BALB/cJ mice (.pdf)

Experimental allergic encephalomyelitis

• Compound efficacy testing in a model of multiple sclerosis
• Model: SJL/J females administered with proteolipid (PLP)
• Readout: Disease activity scoring
• Sample study: Experimental Autoimmune Encephalomyelitis (EAE) sample study (.pdf)

Delayed type hypersensitivity

• Compound efficacy testing in a model of T cell inflammatory responses
• Model: Topical treatment of BALB/cJ with DNFB
• Readouts:
  • Ear edema
  • Histopathology

Rheumatoid arthritis

• Compound efficacy testing for arthritis
• Models:
  • Collagen peptide injections in DBA/1J
  • Anti-collagen antibody cocktail in BALB/cJ
• Readouts:
  • Ankle and paw swelling
  • Clinical signs of disease (redness, stiffness)
  • Histological scoring of joints
• Sample studies:
  • Collagen-Induced Arthritis sample study (.pdf)
  • Collagen Antibody-Induced Arthritis sample study (.pdf)

Inflammatory bowel disease

• Compound efficacy testing in diverse mouse models of IBD
• Induced models:
  • DSS in C57BL/6J (acute or chronic)
  • TNBS in BALB/cJ (acute)
  • Adoptive transfer of CD45RBhi T cells into immunodeficient mice
• Readouts:
  • Body weight
  • Disease activity index (stool consistency, presence of blood, rectal prolapse)
  • Colon and cecum histopathology
• Sample studies:
  • Acute DSS-Induced IBD Protocol (.pdf)
  • Chronic DSS-Induced IBD Protocol (.pdf)
  • TNBS-Induced IBD Protocol (.pdf)
  • T cell Adoptive Transfer Protocol (.pdf)
  • IBD model comparison (.pdf)

Systemic Lupus Erythematosus

• Compound efficacy testing in mouse lupus models
• Spontaneous models:
  • MRL/MpJ-Fas^lpr/J
  • NZBWF1/J
• Readouts:
  • Creatinine and BUN
  • Kidney histopathology
  • Proteinuria
  • Survival
  • Serum lg
• Sample studies:
  • The MRL-lpr/lpr Mouse model of Systemic Lupus Erythematosus (.pdf) 
  • The NZBWF1 Mouse model of Systemic Lupus Erythematosus (.pdf) 
• Ready to sponsor a study? Request a quote now

Type 1 diabetes

• Compound efficacy testing in spontaneous and induced T1D models
• Models:
  • NOD/ShiLtJ (spontaneous)
  • STZ-treatment (induced)
• Readouts:
  • Plasma insulin
  • Plasma glucose
  • Pancreatic histology
  • β cell loss
• Sample study: Evaluation of Cell Replacement Therapy for Type 1 Diabetes in Mice (.pdf)

Graft-versus-host disease

• Compound efficacy testing in a model of transplant rejection
• Model: NSG mouse engrafted with human peripheral blood mononuclear cells
• Readouts:
  • Survival
  • Tissue-specific inflammatory activity
• Sample study: Allograft Rejection sample study (.pdf)

In vivo pharmacology services facility

JAX^® In Vivo services are performed in our West Sacramento, California facility. We would be happy to arrange a tour.

• Contact an In Vivo testing expert at jaxservices@jax.org
• Call toll free: 1-800-422-6423 (US, Canada & Puerto Rico) or 1-207-288-5845 (from any location)
• Get started using our JAX^® Services request form

Humanized NSG mice

The ideal platform for studying hematopoiesis, inflammatory disease and human-specific pathogens

Spotlight

• A letter from Dr. Neal Goodwin, Director of Business Development, In VivoPharmacology Services
• Listen to Future Pharmaceuticals' podcast interview with Dr. Goodwin, "The Exquisite Model" (.mp3)

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Rheumatoid arthritis:
Collagen-induced arthritis

Collagen antibody-induced arthritis in BALB/cJ (000651) mice. Hematoxylin and eosin (H&E) histological examination of an inflamed ankle joint with multifocal cartilage erosions accompanied by marked inflammatory infiltrates (above) compared to normal (below).

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Inflammatory bowel disease:
Normal vs. DSS-treated colon

Selected images from H&E stained slides from the proximal segment of formalin-fixed colons. These images were taken from the same region of the proximal colon at 100X magnification. Inflammation, crypt abscesses and hyperplasia are evident in DSS-treated mice (below).