Histology service stain/fixative matrix
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The table below displays information on which tissue stains may be used in combination with particular fixatives.
A check mark (√) indicates that a fixative and stain are appropriate.
| Stain | 10% NBF | 4% PFA | Bouins | Telly's |
| LFB/CV (luxol fast blue/cresyl violet | √ | √ | √ | |
| Masson’s Trichrome | √ | √ | √ | √ |
| AF (Aldehyde Fuchsin) in combination with H&E (AF/H&E) | √ | √ | √ | |
| PAS (periodic acid schiff) | √ | √ | √ | √ |
| GMS (Grocott’s methenamine silver) | √ | √ | √ | √ |
| Modified Brown & Hopps (for gram-/+ bacteria) | √ | √ | √ | √ |
| Kinyoun’s Acid Fast Bacteria stain | √ | √ | √ | √ |
| Giemsa (for tissue) | √ | √ | √ | √ |
| Mucicarmine | √ | √ | √ | √ |
| Alcian Blue | √ | √ | √ | √ |
| Toluidine Blue (for mast cells) | √ | √ | √ | √ |
| Gomori’s Method (for iron) | √ | √ | √ | √ |
| Congo Red | √ | √ | √ | √ |
