JAX® Embryo Cryopreservation Services
Speed embryo cryopreservation
Rapid cryopreservation of embryos from genetically engineered mice
Key benefits
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Saves resources when colonies are not in use
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Limits genetic drift and prevents spontaneous loss of phenotype
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Protects valuable mouse strains against disasters
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Facilitates quick recovery of specific and opportunistic pathogen-free (SOPF) mice
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Typically completed four to six weeks after importation
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Requires only three males
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Facilitates strain distribution to fellow researchers
Details
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Available for strains on C57BL/6J, FVB/NJ, DBA/2J, BALB/cByJ, or NOD/ShiLtJ backgrounds
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At least 250 two-cell embryos/strain cryopreserved in 10 straws (25 embryos/straw)
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Cryopreserved embryos are heterozygous (if homozygous males are supplied) or heterozygous and wild-type (if heterozygous males are supplied)
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Quality control: systematic checks throughout cryopreservation process
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Test thaws of control embryos
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IVF of control embryos to validate the media and environmental conditions
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Attempt to recover live born mice (except for strains on C57BL/6J backgrounds)
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Embryos cryopreserved for five years in three tanks at two sites
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Additional years of storage available for $175/strain/year
Pricing
| JAX Mice Strain | Price | # of embryos stored after QCLB |
|---|---|---|
| C57BL/6J | $3,250 | 250 |
| FVB/NJ | $4,350 | 315 |
| DBA/2J | $4,350 | 315 |
| NOD/ShiLtJ | $4,350 | 270 |
| BALB/cByJ | $4,500 | 315 |
Custom embryo cryopreservation
For those strains that require homozygous cryopreservation
Key benefits
- Preserves the inbred or homozygous characteristics of your mouse strain
- Ideal for strains with multiple genetic mutations
- Enables you to eliminate a colony when it is not under active study
- Protects valuable mouse strains against loss due to disaster
- Facilitates quick recovery of specific and opportunistic pathogen-free (SOPF) mice
- Helps you distribute your strains to other researchers
Details
- Best suited for inbreds, homozygotes, or strains with multiple genetic mutations
- Total number of embryos cryopreserved determined by a test cryopreservation and recovery
- Typical timeframe of four to six months
- Requires four to six males and six to ten females from your colony
Pricing
By quotation only. Please contact JAX® Services for more information.
Fees for optional services are as follows:
- Longer storage: $175/strain/year
- QC to live born: $650/strain
- Shipping straws: $295/location
- Shipping litters from two successful transfers: $950
