JAX® Embryo Cryopreservation Services
Speed embryo cryopreservation
Rapid cryopreservation of embryos from genetically engineered mice
Key benefits
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Saves resources when colonies are not in use
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Limits genetic drift and prevents spontaneous loss of phenotype
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Protects valuable mouse strains against disasters
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Facilitates quick recovery of specified and opportunistic pathogen free (SOPF) mice
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Typically completed four to six weeks after importation
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Requires only three males
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Facilitates strain distribution to fellow researchers
Get started using our speed embryo cryopreservation form
Details
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Available for strains on C57BL/6J, FVB/NJ, DBA/2J, BALB/cByJ, or NOD/ShiLtJ backgrounds
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At least 250 two-cell embryos/strain cryopreserved in 10 straws (25 embryos/straw)
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Cryopreserved embryos are heterozygous (if homozygous males are supplied) or heterozygous and wild-type (if heterozygous males are supplied)
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Quality control: systematic checks throughout cryopreservation process
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Test thaws of control embryos
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IVF of control embryos to validate the media and environmental conditions
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Attempt to recover live born mice (except for strains on C57BL/6J backgrounds)
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Embryos cryopreserved for two years in three tanks at two sites
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Additional years of storage available for $190/strain/year
Pricing
Pricing for IVF embryo cryopreservation
| Strain | Stock number | Price* | QC to live-born required? | Total price for service |
|---|---|---|---|---|
| C57BL/6J | 000664 | $3,000 | No | $3,000 |
| FVB/NJ NOD/ShiLtJ DBA/2J |
001800 001976 000671 |
$3,500 | Yes, $650 | $4,150 |
| BALB/cByJ | 001026 | $3,700 | Yes, $650 | $4,350 |
* Includes two years of storage
Pricing for embryo cryopreservation related services
| Description | Price |
|---|---|
| Shipment of straws (first strain) |
$295 |
| Shipment of straws (additional strains in same shipper) |
$40/strain |
| Embryo recovery: Litters from two successful embryo transfers |
$1,475* |
| Extra year of cryo storage (per strain) |
$190/year |
*Price for strains cryopreserved by JAX. All others, please inquire.
Custom embryo cryopreservation
For those strains that require homozygous cryopreservation
Key benefits
- Preserves the inbred or homozygous characteristics of your mouse strain
- Ideal for strains with multiple genetic mutations
- Enables you to eliminate a colony when it is not under active study
- Protects valuable mouse strains against loss due to disaster
- Facilitates quick recovery of SOPF mice
- Helps you distribute your strains to other researchers
Get started using our custom embryo cryopreservation form
Details
- Best suited for inbreds, homozygotes, or strains with multiple genetic mutations
- Total number of embryos cryopreserved determined by a test cryopreservation and recovery
- Typical timeframe of four to six months
- Requires four to six males and six to ten females from your colony
Pricing
By quotation only. Please contact JAX® Services for more information.
